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Note: For safety and biohazard guidelines, refer to the. “Safety” appendix in the Applied Biosystems ViiA™ 7 Real-. Time PCR System User Guide (PN 4442661) Sample & Assay Technologies. Life Technologies® ViiA®7 (for ViiA7 Software version 1.2) instrument setup instructions for. qBiomarker Copy Number PCR If the ™ complete the calibration run failed, troubleshoot the failed calibration as described in the Applied Biosystems ViiA 7 Real- Time PCR System User Guide 14 Apr 2011 Contents. 5. Applied Biosystems ViiA™ 7 Real-Time PCR System User Guide: Calibration, Maintenance, Networking, and Security. About This Guide . 12 About This Guide Purpose The Applied Biosystems ViiA 7 Real-Time PCR System User Guide provides reference information for the ViiA 7 Instrument and Each booklet in this guide provides a tutorial for running an example experiment using ViiA™ 7 Software and the example data provided on the installation CD. 10 Apr 2013 ViiA™ 7 Real-Time PCR System. 3 Intuitive software (similar to StepOnePlus .. Single/Multiple peak and Auto/Manual analysis options. conditions for most SNP assays will be as described in this manual (section 4), In the Viia7 software, a pre-read (before the thermal cycle) and a post-readmanual for the calibration procedures if needed). ? Please make note of the installed ViiA 7 Software version on your instrument. Procedure. Setting up a PCR