Trimmomatic tutorial on excel "338"

Contact. Please feel free to post a question on the Microbiome Helper google group if you have any issues.; Other technical questions and bug reporting about this repository and tutorials can be sent to gavin.douglas@dal.ca, and questions about the wet-lab protocols can be sent to andre.comeau@dal.ca.

Pre-process the reads (trimmomatic - we're going to skip this step) Align the reads to a reference genome (bowtie2) Call peaks on the aligned reads using condition and control alignments (macs) Investigate the location of the peaks for enriched annotation and go terms (homer) Extra: Investigate the content of the peaks for binding motifs (homer) Trimmomatic performs a variety of useful trimming tasks for illumina paired-end and single ended data.The selection of trimming steps and their associated parameters are supplied on the command line.. Availability and Restrictions Versions. The following versions of Trimmomatic are available on OSC clusters: A Galaxy-based bioinformatics pipeline for optimised, streamlined microsatellite development from Illumina next-generation sequencing data Sarah M. Grif?ths1 • Graeme Fox1 • Peter J. Briggs2 • Ian J. Donaldson2 • Simon Hood3 • Pen Richardson3 • George W. Leaver3 • Nathan K. Truelove4 • Richard F. Preziosi1 Trimmomatic is a flexible read trimming tool for Illumina NGS data. "Trimmomatic performs a variety of useful trimming tasks for illumina paired-end and single ended data.The selection of trimming steps and their associated parameters are supplied on the command line." Tutorial: Metagenomic Classification with Kraken. In this tutorial we will learn how to classify our metagenomic reads obtained with Illumina sequencing platform with Kraken, a system for assigning taxonomic labels to short DNA sequences. Quality Control of Illumina Data at the Command Line Quick UNIX Introduction: UNIX is an operating system like OSX or Windows. The interface between you and the UNIX OS is called "the shell". There are a few