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STANDARD OPERATING PROCEDURE FOR MYCOBACTERIOLOGY LABORATORY This Standard Operating Protocol (SOP) manual is a guideline intended to assist as a 49. DST for FLD and SLD by MGIT 960 Dr. N. S. Gomathi 119 50. NC006_Laboratory Manual Myco Labs_Master_1.0_20150118 20150228.docx Pg. 1 of 101 LABORATORY MANUAL MYCOBACTERIOLOGY - LABORATORIES Protocol Title: A Phase 3, Open-Label Partially Randomized Trial to Evaluate the Efficacy, Safety and Tolerability of the Combination of Moxifloxacin plus PA-824 plus Pyrazinamide after 4 and 6 months in The BD BACTEC MGIT 960 system is for high-volume mycobacteria growth, detection and susceptibility testing and is designed to meet the needs of medium- and high-volume laboratories. Onboard incubation. Safe and efficient onboard incubation eliminates sharps, and the need to handle or transfer monitoring in the BACTEC MGIT 960 (Becton Dickinson) but the older manual system requires tubes to be examined for ? uorescence under a Wood's lamp or with some other long wave UV light source. Though requiring more technical time, the manual system is a lot more affordable for laboratories with a smaller budget. In this The objective of the study was to compare the manual Mycobacteria Growth Indicator Tube (MGIT) method and the BACTEC MGIT 960 system to the BACTEC 460 method for susceptibility testing of The MGIT (mycobacteria growth indicator tube) is a ready-to-use liquid medium produced by Becton Dickinson . The BACTEC MGIT 960 system uses an oxygen-quenching fluorescent sensor technology (BBL MGIT) in conjunction with unique algorithms to determine positivity of the culture tubes. MGIT stands for Mycobacteria Growth Indicator Tube, and 960 indicates the total number of culture tubes it can hold at any given time The BACTEC MGIT 960 System is an in vitro diagnostic instrument for rapid detection of Mycobacteria in clinical specimens other than blood. The average turnaround times were 6.4 days for the MGIT 960 system, 6.5 for

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